PBMCs – Peripheral Blood mononuclear cells – are a mixture of many subsets of lymphocytes and monocytes. The isolation of PBMCs is fundamental for immunology research.
HiSep™ Lymphocyte Separation Media are sterile, ready to use, density gradient media for isolating viable mononuclear cells from human blood and other animal species. They are made up of a polymer of sucrose with high synthetic molecular weight. Mononuclear cells (lymphocytes and monocytes) and platelets are contained in the banded plasma-LSM interphase due to their density and the pellet that is formed contains mostly erythrocytes and granulocytes, which have migrated through the gradient to the bottom of the tube.
Code | Product Name | Density (g/ml) | Cell type separated |
---|---|---|---|
LS001 | HiSep™ LSM 1077 | 1.077 | Human mononuclear cells (Lymphocytes and monocytes) |
LS002 | HiSep™ LSM 1073 | 1.073 | Lower density human mononuclear cells (eg- mesenchymal stromal cells or monocytes). |
LS003 | HiSep™ LSM 1084 | 1.084 | Mononuclear cells of higher density from Chicken, Rats, Mice and other Mammals) |
LS004 | GranuloSep™ GSM1119 | 1.119 | Human mononuclear cells and granulocytes |
LS005 | Ficoll 400-20% | Commonly used to prepare density gradients | |
LS006 | HiSep™ LSM 1077 with 10mM HEPES | 1.077 | Human mononuclear cells (Lymphocytes and monocytes) |
Purity and viability are two major criteria for the performance of Separation media. Hence, both are controlled very strictly for each batch including use of Flow Cytometry and CASY counting and volume-based size detection.
Flow cytometric analysis showing lymphocytes and monocytes gated populations based on forward scatter (FSC) and side scatter (SSC).
PBMC sample analyzed using CASY cell counter & analyzer showing
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[LS001] HiSepTM LSM 1077 is an iso-osmotic, low viscosity medium containing polysucrose and diatrizoic acid dihydrate. This medium offers a quick and reliable method for the simple isolation of human mononuclear cells and lymphocytes from defibrinated EDTA or heparin treated human blood
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[LS002] HiSepTM LSM 1073 is a solution of polysucrose and sodium diatrizoate. This medium offers a quick and reliable method for the simple isolation of lower density mononuclear cells from human peripheral blood, umbilical cord blood and bone marrow
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[LS003] HiSepTM LSM 1084 is a solution of polysucrose and sodium diatrizoate. This medium offers a quick and reliable method for the simple isolation of mononuclear cells from blood from chicken, rats, mice and other mammals
Price on request
[LS004] GranuloSepTM GSM 1119 is a solution of polysucrose and sodium diatrizoate. When combined with HiSep™ LSM 1077, it permits separation of mononuclear cells and granulocytes from defibrinated EDTA or heparin treated human blood
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